What is SPE HPLC?
Solid phase extraction (SPE) is a technique designed for rapid, selective sample preparation and purification prior to the chromatographic analysis (e.g. HPLC, GC, TLC). In SPE, one or more analytes from a liquid sample are isolated by extracting, partitioning, and/or adsorbing onto a solid stationary phase.
What is the process of SPE?
Solid-phase extraction (SPE) is an extractive technique by which compounds that are dissolved or suspended in a liquid mixture are separated from other compounds in the mixture according to their physical and chemical properties.
How do you choose a SPE?
The general method of SPE is: use the principle of chromatographic analysis of selective adsorption and selective elution, pass the liquid sample through the adsorbent, retain the analytes in it, wash the impurities with a suitable solvent, and then elute the analytes with a small amount of solvent, to achieve the …
Why is SPE used?
Solid-phase extraction (SPE) is a sample preparation technique routinely used in analytical laboratories for the extraction of analytes from a complex matrix. This sample preparation technique enables the extraction, cleanup and concentration of analytes prior to their quantification.
What is SPE column?
The CHROMABOND solid phase extraction (SPE) columns help you to be successful in the laboratory. Our products enable you to clean‑up the sample and to enrich the analytes. This is how you achieve high quality results for your in analysis. Read more. Liquid-liquid extraction (LLE), supported/solid liquid extraction(SLE)
What means SPE?
SPE
| Acronym | Definition |
|---|---|
| SPE | Society of Petroleum Engineers |
| SPE | Solid Phase Extraction |
| SPE | Strategic Planning and Evaluation (US FEMA) |
| SPE | Sony Pictures Entertainment |
What form of SPE is most commonly used?
Most commonly, SPE is practiced using miniature column or cartridge devices. An example is shown here. A mixture of three dyes is loaded onto the cartridge in a weak solvent, causing strong sample retention in a narrow band that appears black at the column inlet.
How do you use SPE?
To perform normal-phase chromatography with SPE cartridges, use a step gradient of nonpolar solvents with a polar packing material.
- Condition the cartridge with six to ten hold-up volumes of non-polar solvent, usually the same solvent in which the sample is dissolved.
- Load the sample solution onto the cartridge bed.
How do I use the SPE method development tool?
This SPE Method Development Tool will recommend an extraction method for you in under 1 minute! To start, enter the name of the analyte you’d like to extract or select “proprietary” to develop a method based on the chemical characteristics of your analyte. Like this tool?
How do you use a SPE cartridge for normal phase chromatography?
To perform normal-phase chromatography with SPE cartridges, use a step gradient of nonpolar solvents with a polar packing material. Condition the cartridge with six to ten hold-up volumes of non-polar solvent, usually the same solvent in which the sample is dissolved. Load the sample solution onto the cartridge bed.
How to choose sorbent and eluents for solid-phase extraction?
Keep in mind that solid-phase extraction has the same fundamental basis as HPLC. Any knowledge of the chromatographic behavior of the analytes of interest, and of other matrix components, can help in choosing the proper sorbent and eluents.
How to develop GC-MS method?
GC Method Development 1 This approach is equally applicable to GC-MS. 2 Verify analyte solubility and select a suitable solvent / sample prep for samples. 3 Select a suitable method of detection. 4 Review existing applications to give indication of preliminary conditions.