What is rhodamine fluorescence?
Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a staining fluorescent dye.
What is the role of Rhodamine 123?
Rhodamine 123 is a cell-permeant, cationic, green-fluorescent dye that is readily sequestered by active mitochondria without cytotoxic effects. This product has been used to assay mitochondrial membrane potential in populations of apoptotic cells.
Does rhodamine have fluorescence?
Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.
Why is rhodamine fluorescence?
They are the chromophores and fluorophores of rhodamine dyes responsible for the strong light absorption and fluorescence emission in the visible region. One advantage of these dyes over rhodamines is the absence of lactone form due to the absence of the carboxyphenyl moiety.
What is the chemical formula of Rhodamine B?
C28H31ClN2O3
Rhodamine B/Formula
Is rhodamine a base or acid?
Rhodamine B base is a colorless dye with undetectable fluorescence feature. In acidic condition, rhodamine B base gets transformed into a colored rhodamine dye that acts as a stable and efficient laser dye.
What wavelength is rhodamine?
Rhodamine B is a fluorescent compound with an excitation peak at 546 nm and an emission peak at 568 nm.
What color does rhodamine fluorescence?
Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with fluorometers….Rhodamine B.
| Names | |
|---|---|
| Appearance | red to violet powder |
| Melting point | 210 to 211 °C (410 to 412 °F; 483 to 484 K) (Decomposes) |
| Solubility in water | 8 to 15 g/L (20 °C) |
| Hazards |
Is rhodamine B cationic or anionic?
In addition, other cationic dyes, like malachite green (MG) and rhodamine B (RhB), as well as anionic dyes, like indigo carmine (IC), acid fuchsine (AF), acid orange (AO), and fluorescein disodium salt (FD), are also tested in this work.
What is rhodamine B dye?
Rhodamine B /ˈroʊdəmiːn/ is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with fluorometers.
What color is rhodamine?
Rhodamine Red Color Codes
| Hex Code | #E44C9A |
|---|---|
| Closest Web Safe | #CC3399 |
| Inverse Color | #1BB365 [Green (Crayola)] |
| Pantone® | 7424 C |
| Closest RAL | 4003 [Heather violet] |
What is rhodamine used for?
What is the function of rhodamine 123 in flow cytometry?
Rhodamine 123 is a fluorescent dye most commonly used in flow cytometry as functional reporter for P-glycoprotein (Pgp). It is part of the rhodamine family of flurone dyes. The dye is mostly used to assess mitochondrial bioenergetics in living cells.
Why is rhodamine 123 not staining in human hepatic cells?
HSCs show low-staining with a mitochondrial fluorescent dye, Rhodamine 123.29 This is because of the multidrug-resistance 1a/b (MDR1a/b) gene expressed in HSCs.
How is mitochondrial loading with rhodamine 123 performed?
Mitochondrial loading with rhodamine 123 is performed by washing cultures in situ once with PBS, and then incubating the cultures with 5 μ g/ml rhodamine 123 in serum-free medium (prepared from 2 mg/ml stock in ethanol). Incubation time is based on kinetic analysis of rhodamine 123 loading, which is typically 30 minutes in astroglia.
What is the best way to store rhodamine 123?
Storage:Rhodamine 123 should be stored at +4°C (L) and protect from light and moisture. Dihydrorhodamine 123 and the salt form should be stored at –20°C under nitrogen or argon and protect from light and moisture P.1 FluoProbes® FT-AM352A