What is p53 mutation status?
p53 mutation is the most common genetic abnormality found in. human cancer.12 In cell lines, loss of p53 activity is usually linked. with several specific landmarks such as defect in growth arrest or. apoptosis after DNA damage and lack of induction of p53-regulated.
Which one of the following is a human breast tumor cell line?
Cell Lines: The most studied is MCF-7, an estrogen receptor (ER)-positive cell line derived from a pleural effusion in a patient with breast cancer. Other ER-positive cell lines include ZR-75-1 and BT-474.
What is a normal cell line?
An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cells can therefore be grown for prolonged periods in vitro.
What is MCF7 cell line?
MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26]. It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI [27].
Is p53 mutated in HeLa?
Endogenous HeLa p53 proteins are easily detected in HeLa cells transfected with mouse deletion mutant p53 gene. Oncogene.
What is the history of bt-549?
The BT-549 line was isolated in 1978 by W.G. Coutinho and E.Y. Lasfargues. Source tissue consisted of a papillary, invasive ductal tumor which had metastasized to 3 of 7 regional lymph nodes.
Is the bt-549 a suitable transfection host?
This cell line is a suitable transfection host. The BT-549 line was isolated in 1978 by W.G. Coutinho and E.Y. Lasfargues. Source tissue consisted of a papillary, invasive ductal tumor which had metastasized to 3 of 7 regional lymph nodes. modal number = 78; range = 73 to 80.
How can I stain bt-549 cells with anti-CLPB antibody?
Immunofluorescence staining of HEK-293 and BT-549 cells with anti-CLPB antibody co-localized with the mitochondrial staining using a MitoTracker dye.