What is a synthetic oligo?

What is a synthetic oligo?

Abstract. Synthetic oligonucleotides (ODNs) are short nucleic acid chains that can act in a sequence specific manner to control gene expression.

How do you resuspend RNA oligo?

Tips for resuspending and diluting your oligonucleotides

1. During the dry-down process, oligos form a white flakey pellet at the bottom of the tube.
2. If resuspension is difficult, try heating the oligo at 55°C for 1–5 minutes, then vortex thoroughly.
3. IDT oligonucleotides (both DNA and RNA) are typically shipped dry.

Are oligos and primers the same thing?

The term oligonucleotide is derived from the Greek “oligo,” which means few or small. Oligonucleotides made up of 2′-deoxyribonucleotides are the molecules used in polymerase chain reaction (PCR). These are referred to as primers and are used to massively amplify a small amount of DNA.

What are oligo probes?

Oligonucleotide probes are short stretches of single-stranded DNA or RNA used to detect the presence of complementary nucleic acid sequences (target sequences) by hybridization. Oligonucleotide probes are usually labelled, for example with radioisotopes, epitopes, biotin or fluorophores to enable their detection.

How does an oligonucleotide work?

Abstract. Antisense oligonucleotides (AS ONs) are synthetic DNA oligomers that hybridize to a target RNA in a sequence-specific manner. They have successfully been employed to inhibit gene expression, modulate splicing of a precursor messenger RNA, or inactivate microRNAs.

Can you use water instead of TE buffer?

that are easily reproducible or you don’t need them for longer time, you can just use water! The pH of TE buffer is slightly basic however water have slightly acidic pH. This basic pH of TE buffer makes DNA more soluble and EDTA helps to protect from the DNase as Ariane Eberhardt said.

Is it OK to vortex primers?

Don’t vortex too much. But you can vortex gently for 5-10 for proper mixing. It would not break primers.

What is an oligo dT primer?

Description. Oligo (dT)18 Primer is single-stranded sequence of deoxythymine (dT), used for priming reactions catalysed by reverse transcriptase. The transcript is primed in the poly(A) tail of mRNA molecules.

What is this oligonucleotide probe specifically called as?

Briefly, a pool of oligonucleotides probes (DNA or RNA) is synthesized to selectively hybridize to the targeted exonic regions of genomic DNA.

How do oligonucleotide probes work?

Why choose our oligos?

Our oligos are made to your specifications, with rigorous quality control, and quick turnaround for use in a variety of applications, including PCR, cloning, sequencing, and gene detection. Use the improved ordering portal to place your orders.

What is the use of oligo 12 18 primer?

Oligo(dT)12-18 Primer is suitable for use in first-strand cDNA synthesis with reverse transcriptase. The primer hybridizes to the poly(A) tail of mRNA. It is phosphorylated on the 5´ end to facilitate cloning of cDNA.

What is Thermo Fisher Scientific custom DNA oligos?

Custom DNA Oligos | Thermo Fisher Scientific – US Custom DNA Oligos Synthesis Services Our oligos are made to your specifications, with rigorous quality control, and quick turnaround for use in a variety of applications, including PCR, cloning, sequencing, and gene detection. Use the improved ordering portal to place your orders.

What is an anchored oligo (dT) primer?

Anchored Oligo (dT) primers have 2 random bases at the 3′ end of the stretch of Ts. The first random base is either an A, G, or C, while the second can be any of the 4 standard nucleotides, i.e., A, T, G or C. The use of anchored oligo (dT) primers results in increased cDNA synthesis yields. View more ›.