How do you use an RNAlater solution?
How do I use RNAlater solution? Trim the tissue to be less than 0.5 cm in at least one dimension and simply submerge it in 5 volumes of RNAlater solution (e.g., a 0.5 g sample requires about 2.5 mL of RNAlater solution). Small organs such as rat kidney, liver, and spleen can be stored whole in RNAlater solution.
How long does tissue stay in RNAlater?
Place the fresh tissue in 5–10 volumes of RNAlater® Solution. Most samples in RNAlater® Solution can be stored at room temperature for 1 week without compromising RNA quality, or at –20°C or –80°C indefinitely.
Is RNAlater compatible with trizol?
Tissue stored in RNA later is compatible with all commonly used RNA isolation methods, including single reagent isolation products like Trizol®, and all of Ambion’s RNA isolation kits. It is also possible to extract both genomic DNA and total protein from samples stored in RNA later.
What is the composition of RNAlater?
Actually, RNALater is just a near-saturated Ammonium Sulfate solution buffered with Sodium Citrate, and with some EDTA for metal chelation. Essentially, it causes protein aggregation (mostly reversible) when added to a tissue or cell suspension.
Does RNAlater expire?
Once in RNAlater, samples can be stored for up to 1 day at 37°C, 1 week at 25°C, 1 month or more at 4°C, and long-term at -20°C or -80°.
Does RNAlater preserve DNA?
Thus, RNAlater successfully preserved both RNA and DNA for up to 1 month at room temperature, and up to 4 months at 5°C, providing an alternative to the deep freezing. This method will enable a greater integration of molecular methods in ecological studies.
Does RNAlater stop transcription?
Thus, the rate of tissue infiltration allows a short-term alteration in transcription, but the RNAlater successfully inhibits the translational machinery before a substantial amount of protein is produced or degraded.
How much RNAlater do cells use?
1) How do I use RNAlater to store my tissue/cell sample? For fresh tissue, simply cut samples to a maximum thickness of 0.5 cm in any one dimension and submerge in 5 volumes of RNAlater. Cultured cells should be pelleted, resuspended in a small volume of PBS, then mixed with 5-10 volumes of RNAlater.
Does RNAlater fix cells?
No, the tissue is not fixed, and RNAlater does not really fix it, it’s a RNA protection agent.
What is RNAlater stabilization solution?
Invitrogen RNAlater Stabilization Solution is an aqueous, nontoxic tissue storage reagent that rapidly permeates tissues to stabilize and protect cellular RNA. RNAlater solution minimizes the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing.
Which RNA isolation methods are compatible with RNAlater solution?
Compatibility of various RNA isolation methods with RNAlater solution. Freshly dissected whole mouse liver and heart were placed in RNAlater solution and stored at 4°C for three days. RNA was isolated from equal mass amouts of each tissue using Ambion’s ToTALLY RNA, RNAqueous, or Poly (A)Purist kits.
How do you dispose of Rn alater solution?
Disposal of RNAlater Solution RNAlater Solution can be safely discarded down the sink and flushed with water. Chemical reactivity with oxidants WARNING RNAlater Solution is known to react with hypochlorite solu-tions, such as common bleach. The reaction releases toxic chlo-rine gas, and is violent enough to generate heat. Similar
How much RNAlater do I need to use for tissue trimming?
Trim the tissue to be less than 0.5 cm in at least one dimension and simply submerge it in 5 volumes of RNAlater solution (e.g., a 0.5 g sample requires about 2.5 mL of RNAlater solution).