What is the function of DNA polymerase I and III?
In Escherichia coli, five DNA polymerases have been found and designated as DNA polymerase I–V, in order of their discovery. The main function of the third polymerase, Pol III, is duplication of the chromosomal DNA, while other DNA polymerases are involved mostly in DNA repair and translesion DNA synthesis.
What are the functions of RNA polymerase I II and III?
RNA polymerase I (RNAPI) transcribes rRNA genes, RNA polymerase II (RNAPII) transcribes mRNA, miRNA, snRNA, and snoRNA genes, and RNA polymerase III (RNAPIII) transcribes tRNA and 5S rRNA genes. This is in contrast with prokaryotes where a single RNA polymerase is responsible for the transcription of all genes.
What is the function of DNA polymerase 1?
Abstract. DNA polymerase I (pol I) processes RNA primers during lagging-strand synthesis and fills small gaps during DNA repair reactions.
What are the functions of DNA Pol III and III in prokaryotes?
Table 14.4. 1: Prokaryotic DNA Replication: Enzymes and Their Function
| Enzyme/protein | Specific Function |
|---|---|
| DNA pol I | Exonuclease activity removes RNA primer and replaces with newly synthesized DNA |
| DNA pol II | Repair function |
| DNA pol III | Main enzyme that adds nucleotides in the 5′-3′ direction |
What is the function of DNA polymerase III quizlet?
DNA polymerase III adds DNA nucleotides to the primer(s), synthesizing the DNA of both the leading and the lagging strands.
How does DNA polymerase 3 work?
Primase synthesizes RNA primers complementary to the DNA strand. DNA polymerase III extends the primers, adding on to the 3′ end, to make the bulk of the new DNA. RNA primers are removed and replaced with DNA by DNA polymerase I. The gaps between DNA fragments are sealed by DNA ligase.
What is the difference between RNA polymerase I and II?
The main difference between RNA Polymerase 1, 2 and 3 is that the RNA polymerase 1 (Pol 1) transcribes rRNA genes and, the RNA polymerase 2 (Pol 2) mainly transcribes mRNA genes while the RNA polymerase 3 (Pol 3) mainly transcribes tRNA genes.
What is the function of DNA polymerase 2?
These polymerases all function to proofread the newly synthesized DNA in the 3′→5′ direction. These polymerases are capable of synthesizing DNA on both the leading and lagging strands. This class of polymerase tends to be very accurate which allows them to correct any mispairings that occur during DNA synthesis.
What is the function of DNA polymerase 3 quizlet?
What is the function of DNA polymerase II?
What is the function of polymerase II?
RNA polymerase II (Pol II) is the central enzyme that catalyses DNA-directed mRNA synthesis during the transcription of protein-coding genes.
What is the function of DNA polymerase 1 2 and 3?
DNA polymerase 1, 2 and 3 are prokaryotic DNA polymerases involved in DNA replication. Pol 1 catalyzes the repairing of DNA damages. Pol 2 catalyzes the fidelity and processivity of DNA replication. Pol 3 catalyzes the 5’ to 3’ DNA polymerization.
What is the function of DNA polymerase III in E coli?
DNA Polymerase III is the main enzyme for replication in E.coli. It is coded by polC gene. The polymerization and processivity rate is maximum in DNA polymerase III. It also has proofreading 3’→5’ exonuclease activity.
What is the function of Pol II in DNA replication?
DNA pol II is a monomeric enzyme but interacts with accessory subunits of the pol III replicase, the γ – δ clamp loading complex and the β clamp. The clamp tethers the DNA polymerase to the terminus of the growing DNA chain.
What type of enzyme is Pol2?
DNA polymerase 2 (Pol 2) is a prokaryotic enzyme which catalyzes the DNA replication. It belongs to the polymerase B family and is encoded by the gen polB. It was first discovered from E Coli by Thomas Kornberg in 1970.