What is Novex gel?

What is Novex gel?

Invitrogen Novex Tris-Glycine Gels are robust gels for a wide range of sample types and molecular weights. They offer sharp straight bands and easier-to-load, larger capacity wells, and can be used for denaturing or native PAGE applications. Find the right gel for your protein Explore welcome packs.

What is 4/12 Bis Tris gel?

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions.

What is a 4/12 gel?

A variety of 4-12% gradient gels can be found from different suppliers for protein electrophoresis. Precast gradient gels are solid, polymerized acrylamide slabs with a gradually increasing concentration toward the bottom of the gel.

What is the purpose of loading protein molecular weight standards on the gel?

A protein MW standard (a collection of proteins of known size) is always run on the gel and used to estimate the sizes of proteins in the other lanes.

How do you use tricine gel?

Add H2O to 500ml total volume. Add 1.5 g SDS and store at 4 C. Samples (~5-10ul of protein prep) should be mixed with sample buffer and boiled for 5 minutes before loading on gel. Run gels at a constant voltage of 100-150 V until the dye front reaches the bottom of the gel.

What is BIS-Tris gel?

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications.

How do I make Bis-Tris gels?

How to Make Your Own Bis-Tris Gels

1. 5X Low MW Running Buffer. 250 mM MES. 250 mM Tris.
2. 5X High MW Running Buffer. 250 mM MOPS. 250 mM Tris.
3. 200X Running Buffer Reducing Agent. 1 M sodium bisulfite. Add to running buffer at 5mM final concentration.
4. 3.5X Gel Buffer. 1.25 M bis-Tris (pH 6.5-6.8 with HCl)

What is NuPAGE?

Additional Information. Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels designed to give optimal separation of a wide range of proteins under denaturing conditions. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications.

What is Native page used for?

Native polyacrylamide gel electrophoresis (PAGE) is most suitable for studying the composition and structure of native proteins, as both their conformation and biological activity will remain intact during the analysis.

What is the meaning of IEF?

IEF

Acronym	Definition
IEF	Investor Education Fund
IEF	International Energy Foundation
IEF	Implied Emission Factor
IEF	International Economy Freight (commerical carriage modality; FedEx)