What is BrdU assay?
Bromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes.
What are BrdU cells?
Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is not a marker of the S-phase of the cell cycle. As a thymidine analog, it is a marker of DNA synthesis.
What does BrdU stain for?
BrdU (Bromodeoxyuridine / 5-bromo-2′-deoxyuridine) is an analog of the nucleoside thymidine used in the BrdU assay to identify proliferating cells. BrdU antibodies can be used in conjunction with cell type markers such as doublecortin, and NeuN to identify proliferating cells and newly differentiated neurons.
How can I detect incorporated BrdU?
Incorporated BrdU can be readily detected with anti-BrdU antibodies, such as the Mouse Anti-BrdU Antibody, clone Bu20a (MCA2483) and Rabbit Anti-BrdU Antibody (AHP2405). However, for successful staining it is important to include a DNA denaturing step to allow the antibody access to the incorporated BrdU.
What does BrdU stand for?
1 Miller School of Medicine, University of Miami, Miami, FL, USA. Bromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes.
What is BrdU labeling and detection protocol?
BrdU Labeling and Detection Protocol DNA synthesis–based cell proliferation assay Cell proliferation can be measured with the thymidine analog BrdU (5-bromo-2’-deoxyuridine) following its incorporation into newly synthesized DNA and its subsequent detection with an anti-BrdU antibody. This protocol can be used for:
How can I control for tissue incorporation of BrdU?
If you need a positive control for tissue incorporation of BrdU, consider BrdU control slides ab129956. BrdU antibodies can be used in conjunction with cell type markers such as doublecortin, and NeuN to identify proliferating cells and newly differentiated neurons.