What are HRP conjugated antibodies?

What are HRP conjugated antibodies?

​​​Horseradish peroxidase (HRP) is a 44 kDa glycoprotein with 6 lysine residues, which can be conjugated to antibodies and proteins for use in a variety of applications. HRP is a popular detection label used in research. Antibody-HRP conjugates are commonly used in ELISA, IHC, and western blotting.

What is the role of goat anti rabbit IgG horseradish peroxidase conjugate in the experiment?

Goat anti-Rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary. Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.

Which antibody isotypes exist as subtypes?

In mammals, antibodies are classified into five main classes or isotypes – IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively.

What are HRP secondary antibodies?

Horseradish Peroxidase (HRP) has a high turnover rate that allows HRP secondary antibodies to generate strong signal in a short time span. HRP secondary antibodies are commonly used in western blot (WB), immunohistochemistry (IHC) and ELISA.

What is goat anti mouse antibody?

Anti-mouse secondary antibodies are generated by immunizing a host animal (e.g., a goat) with a pooled population of immunoglobulins (Ig) from the target species (i.e., mice). These antibodies are designated as cross-adsorbed or highly cross-adsorbed, based on the amount of processing which has occurred.

Why are there different antibody isotypes?

In immunology, antibodies (immunoglobulins (Ig)) are classified into several types called isotypes or classes. The variable (V) regions near the tip of the antibody can differ from molecule to molecule in countless ways, allowing it to specifically target an antigen (or more exactly, an epitope).