What is SPT16?
Standard Name SPT16 1 Systematic Name YGL207W SGD ID SGD:S000003175 Aliases SSF1 , CDC68 20 Feature Type ORF , Verified Description Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by …
What does the fact Complex do?
The FACT Complex Helps to Maintain the Tetrasome Integrity and Facilitates the Deposition of H2A/H2B Dimers into Tetrasomes to Form Nucleosomes by the SSRP1 Subunit. Nucleosome assembly is another important process in maintaining chromatin integrity.
What’s a chromatin kid definition?
Chromatin is a way for DNA to take up less space in the nucleus of the cell. Loose DNA is called chromatin when it becomes tighter; this is done by wrapping itself around structures called histones. Histones are small molecules that look like little balls.
Is FACT a histone chaperone?
FACT is a heterodimeric histone chaperone consisting of the SSRP1 and SPT16 proteins and is conserved among eukaryotes. It interacts with the histones H2A-H2B and H3-H4 as well as with DNA.
What is chromatin quizlet?
Chromatin. An organelle which forms chromosomes during cell division. Consists of DNA, RNA, and proteins. This organelle can have different structures (e.g.an “x” or lines spinning). This organelle is only found in a eukaryote cell.
Is chromatin coiled or uncoiled?
Explanation: Chromatin is unpaired, they are uncoiled, long and thin sturctures inside the nucleus, it can be found throughout the whole cell cycle. Chromosomes are condensed Chromatin Fibers. They are paired, coiled, thick and ribbon-like structure.
What is fact in transcription?
FACT (facilitates chromatin transcription, sometimes facilitates chromatin transactions) is a heterodimeric protein complex that affects eukaryotic RNA polymerase II (Pol II) transcription elongation both in vitro and in vivo. FACT consists of 140 and 80 kilodalton (kDa) subunits.
What is a histone chaperone?
Histone chaperones are the architects of epigenetic inheritance. They are responsible for deposition of histones on DNA to form chromatin, in addition to their removal from DNA to enable genomic processes to occur and be tightly regulated.
What does the chromatin do?
Chromatin is the material that makes up a chromosome that consists of DNA and protein. The major proteins in chromatin are proteins called histones. They act as packaging elements for the DNA. The reason that chromatin is important is that it’s a pretty good packing trick to get all the DNA inside a cell.
What do you mean by chromatin?
Chromatin is a complex of DNA and proteins that forms chromosomes within the nucleus of eukaryotic cells. Each nucleosome is composed of DNA wrapped around eight proteins called histones.
What is coiled and condensed chromatin?
Chromatin fibers are coiled and condensed to form chromosomes. Chromatin makes it possible for a number of cell processes to occur including DNA replication, transcription, DNA repair, genetic recombination, and cell division. People often confuse these three terms: chromatin, chromosome, and chromatid.
How does the spt16-pob3 dimer work?
The SPT16-POB3 dimer weakly associates with multiple molecules of NHP6 (NHP6A or NHP6B) to form the FACT (yFACT or SNP) complex. The FACT complex interacts with the CK2 (casein kinase II) complex subunits CKA1, CKA2, CKB1 and CKB2 and the components of the transcription machinery CHD1, CTR9, PAF1 and CDC73.
How do mutations in spt16/cdc68 affect promoter function in Saccharomyces cerevisiae?
Malone EA, et al. (1991) Mutations in SPT16/CDC68 suppress cis- and trans-acting mutations that affect promoter function in Saccharomyces cerevisiae. Mol Cell Biol 11 (11):5710-7 PMID: 1922073
What is the difference between spt16/ygl207w and SSF1?
Nomenclature conflict: SSF1 has been used in the literature to refer to both SPT16/YGL207W, which encodes a transcription elongation factor and SSF1/YHR066W, which encodes a rRNA binding protein. Malone EA, et al. (1991) Mutations in SPT16/CDC68 suppress cis- and trans-acting mutations that affect promoter function in Saccharomyces cerevisiae.