What does BrdU measure?
Bromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes.
What color is BrdU?
BrdU Cell Proliferation Assay Kit 6813
| Product Includes | Quantity (with Count) | Solution Color |
|---|---|---|
| BrdU | 1 x 150 µl | |
| Fixing/denaturing Solution | 2 x 25 ml | |
| BrdU Mouse Detection mAb | 1 x 500 µl | Green |
| Anti-mouse IgG, HRP-Linked Antibody | 1 x 500 µl | Red |
What is BrdU and how does it detect mitosis?
Bromodeoxyuridine (BrdU) is a thymidine analog to which monoclonal antibodies have been developed. This methodology allows detection of endothelial cells in the S phase at the light microscopic level but, unlike autoradiography, there is no 3- to 4-week exposure period.
What is BrdU staining?
BrdU (Bromodeoxyuridine / 5-bromo-2′-deoxyuridine) is an analog of the nucleoside thymidine used in the BrdU assay to identify proliferating cells. Print this protocol. BrdU labeling can be performed in vitro for cell lines and primary cell cultures, or in vivo for labeling cells within a living animal.
What is BrdU Labelling?
What is BrdU cells?
Bromodeoxyuridine (BrdU) is a thymidine analog that incorporates DNA of dividing cells during the S-phase of the cell cycle. As such, BrdU is used for birth dating and monitoring cell proliferation. As a thymidine analog, it is a marker of DNA synthesis.
What is the BrdU staining and BrdU assay protocol?
BrdU staining and BrdU assay protocol. BrdU (Bromodeoxyuridine / 5-bromo-2′-deoxyuridine) is an analog of the nucleoside thymidine used in the BrdU assay to identify proliferating cells. Print this protocol. BrdU labeling can be performed in vitro for cell lines and primary cell cultures, or in vivo for labeling cells within a living animal.
How do you label in vivo cells with BrdU?
There are several methods for labeling cells in vivo with BrdU. Two commonly used methods are intraperitoneal injection and oral administration. Dilute BrdU in PBS to make a sterile solution of 10 mg/mL. For mice, as a general rule, inject the BrdU solution to a concentration of 100 mg/kg.
Can BrdU be used to measure cell proliferation?
Measurement of cell proliferation via BrdU incorporation in combination with multicolor cell surface staining would facilitate studies on cell subsets that require multiple markers for their identification.
What is the best way to use a BrdU antibody?
BrdU antibodies can be used in conjunction with cell type markers such as doublecortin, and NeuN to identify proliferating cells and newly differentiated neurons. For rapid, convenient results, we also recommend our BrdU staining IHC kit which contains all the reagents you need for staining cells or tissues which have incorporated BrdU.