What is the histology of the colon?
The colon has the typical histological structure as the digestive tube: mucosa, submucosa, muscularis and serosa/adventitia. The mucosa is lined by simple columnar epithelium (lamina epithelialis) with long microvilli. It is covered by a layer of mucus which aids the transport of the feces.
What is H and E stain explain in detail?
The most commonly used staining system is called H&E (Haemotoxylin and Eosin). H&E contains the two dyes haemotoxylin and eosin. It is used to stain acidic (or basophilic) structures a purplish blue. (Haematoxylin is not strictly a basic dye, but it is used with a ‘mordant’ that makes this stain act as a basic dye.
Does the colon have villi?
The large intestine differs from the small intestine in the following important ways: villi are absent in the large intestine; the microvilli of the large intestine epithelial cells are much less abundant; goblet cells are more prominent in the large intestine; endocrine cells are less prominent in the large intestine; …
What is the histology of the large intestine?
The thick mucosa has deep crypts, but there are no villi. The epithelium is formed of columnar absorptive cells with a striated border, many goblet cells, endocrine cells and basal stem cells, but no Paneth cells. The surface epithelial cells are sloughed into the lumen, and have to be replaced around every 6 days.
Why do mitochondria stain eosinophilic?
Mitochondria stain eosinophilic due to their high protein content and the presence of mitochondrial DNA.
How do you remove hematoxylin stains?
1% Acid Alcohol should do the trick just fine. HCL in 70% ethanol. Rinse either with 0.25% hydrochloric acid (HCl) for 2-5 seconds or 1% acid alcohol (1ml Conc HCl in 100ml ethanol) to remove excess stain from the slide, Then keep the slides in running water for 3 minutes for blueing.
Which cells are found in intestinal lining?
The intestinal epithelium is lined with a single layer of polarized cells, among which the major types include enterocytes, goblet cells, Paneth cells, stem cells, and others.
Does the small intestine have villi?
How can the small intestine digest so much? Villi: The folds form numerous tiny projections which stick out into the open space inside your small intestine (or lumen), and are covered with cells that help absorb nutrients from the food that passes through.
Where are villi found?
the small intestine
The inner wall of the small intestine is covered by numerous folds of mucous membrane called plicae circulares. The surface of these folds contains tiny projections called villi and microvilli, which further increase the total area for absorption.