How does Amido Black work?
Amido Black is a general protein stain. It will react with proteins found in blood, but is not specific for blood. The use of Amido Black results in a dark blue to black staining of the protein that can enhance the contrast and visibility of patterns and impressions and allow for easier documentation.
What does Amido Black react with in fingerprints?
Amido black is an effective blood reagent when latent or difficult to see blood stains are present. AB will develop exceptional fine detail as needed in bloody fingerprints and footwear impressions. Amido black reacts with blood proteins to create a deep blue color change.
What is the purpose of Amido Black in electrophoresis?
Amido black is used to stain proteins on blot transfer membranes. Transferred proteins (>50 ng/band) appear as dark blue bands on a light blue background. Amido black has a sensitivity similar to that of Coomassie blue, but it stains faster.
Is Amido Black reliable?
A new multipurpose cell micro-assay has been developed, using the protein dye amido black 10B as an indicator of cell numbers in 96-well plates. The assay is reliable, rapidly performed and can be combined with morphological evaluation and photography of stained cells.
How do you use Amido Black?
Amido Black Application Procedure:
- Pour sufficient water-based fixing solution to treat the article into a clean, dry, metal dish or pan.
- Immerse the article in the fixing solution for five to six minutes.
- Pour sufficient water-based working solution into a clean, dry, metal dish or pan to treat the article.
How sensitive is Amido Black?
Amido black is very sensitive and works well on non-porous surfaces but its high background color (light to medium blue) compromises contrast on multi-colored porous surfaces. Amido Black is a protein stain, and as such should not be considered as even a presumptive test for blood, let alone a confirmatory test.
What surfaces is Amido Black used on?
Amido black is very sensitive and works well on non-porous surfaces but its high background color (light to medium blue) compromises contrast on multi-colored porous surfaces.
How do you make Amido Black?
Weigh 0.1 g of Amido Black 10B powder dye • Add 40 mL of methanol and 10 mL of glacial acetic acid. Gently mix • Set the volume to 100 mL using distilled (demi) water. Gently mix until all the dye is dissolved. Keep the solution at room temperature for 6 months.
How do you make Amido Black solution?
Amido Black Aqueous Solutions—Field or Lab Use Weigh out 20g of 5-Sulphosalicylic Acid. Place in a clean, dry, 2 liter glass beaker. Measure out 1-liter of Distilled Water. Add to the 5-Sulphosalicylic Acid while stirring with a magnetic stirrer.
What chemicals are used in Amido Black?
Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin….Amido black 10B.
| Names | |
|---|---|
| Chemical formula | C22H14N6Na2O9S2 |
| Molar mass | 616.49 g·mol−1 |
| Appearance | Dark red to black |
| Solubility in water | ~ 30 g/l at 20 °C (68 °F) |
What chemicals are in Amido Black?
Amido black
| PubChem CID | 135436510 |
|---|---|
| Molecular Weight | 595.5 |
| Parent Compound | CID 135416993 (2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-) |
| Component Compounds | CID 5360545 (Sodium) CID 135416993 (2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-) |
How do you make Amido?
Is Amido Black a good blood reagent?
Amido black is an effective blood reagent when latent or difficult to see blood stains are present. AB will develop exceptional fine detail as needed in bloody fingerprints and footwear impressions. Amido black reacts with blood proteins to create a deep blue color change.
What is Amido Black used for in forensic science?
For many years, forensic scientists have used reagents such as Leucomalachite or Ninhydrin to develop blood stains, but both of these substances have a tendency to run off or distort the prints on non-porous surfaces. On the other hand, Amido Black has proven to be quite useful.
What is the best way to apply Amido black?
Nonporous surfaces tend to yield the best results with Amido black and require little or no over stain rinsing. An application using a wash bottle (sold separately) is preferred for the completeness of coverage over spray methods. Lab processing requires the evidence to first be treated with a blood fixative.
Why is Amido black reagent preferred over luminol for photographic documentation?
Its preferred use over luminol is primarily due to its sensitivity and ease of use for photographic documentation. Select a piece of bloodstained brown paper. Dip the paper in the amido black reagent bath for approximately 10 s.
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