How do I use hispur Ni-NTA magnetic beads?

How do I use hispur Ni-NTA magnetic beads?

The HisPur Ni-NTA Magnetic Beads can be used both manually with a magnetic stand as well as with automated platforms such as the Thermo Scientific KingFisher Instruments for high-throughput needs. HisPur Ni-NTA Magnetic Beads are used for small scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins.

What is included in the PBAD/his kit?

The pBAD/His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The vector pBAD/His allows you to express your protein with an N-terminal tag. The vector provides: Three vectors are provided (A, B, and C).

Are polyhistidine magnetic beads immunogenic?

Polyhistidine tags do not typically compromise the biological protein function and are not considered immunogenic, however if cleavage of the His-tag is necessary we recommend using TEV Protease ( NEB# P8112 ). NEBExpress™ Ni-NTA Magnetic Beads yield highly variable binding capacities dependent on the target and conditions.

What does the vector PBAD/his do?

The vector pBAD/His allows you to express your protein with an N-terminal tag. The vector provides: Three vectors are provided (A, B, and C). frame relative to the multiple cloning site to simplify in-frame cloning of your gene.